Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMKKIALFYMGGTFGCIGEPLAPMPYDQFLPQLEKVIP-----PHLTVDCFAAPNIVDSSACTAPDWLRLIQRIQQLQLEGYQHFVVIHGTDTLSYAAATLARFLGQ-SCHIVITGSQYPLLNIQGDNTREFTDAIENLYLALEQVIAL-LVGAYLAFHHQVFHAQTALKTHTTELDAFSGLSSNVEFTPQQNEL----I-VQ--DAQI--EKAASFQLLNWMMQPIATQHLVQQLRHLLPAPPHFLVLQGFGTGNIAVNDEFLATLDELYTRGCVPILATQVTFGGI-DQRYAISTWAKTAKIVINDAHSHADLYAKALQIYLKYPTPEQWLNHWNENLH
2HIM Chain:A ((23-351))KKSIYVAYTGGTIGMQR-----IPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKA-HYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRS-------DGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTAKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADV---VRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMG-----NALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLR


General information:
TITO was launched using:
RESULT:

Template: 2HIM.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1739 -51529 -29.63 -170.62
target 2D structure prediction score : 0.68
Monomeric hydrophicity matching model chain A : 0.71

3D Compatibility (PKB) : -29.63
2D Compatibility (Sec. Struct. Predict.) : 0.68
1D Compatibility (Hydrophobicity) : 0.71
QMean score : 0.428

(partial model without unconserved sides chains):
PDB file : Tito_2HIM.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-2HIM-query.scw
PDB file : Tito_Scwrl_2HIM.pdb: