Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGPDMQAIILDTETHTLNGLPIEIA-YAPIEINAGKLTLDK--SKLFDQLYQVGTPISYAAMAVHHILESDLENQPHYKTFKLPDTTTYIIGHNIDYDIAAIARCGVDVSHIKPICTLALTRKTWPDAEAHNISALIYMISQGSSKARELLKGAHRADAD------IILTANILMHIVYHLNIHDIEELYRVSEEARIPTTINFGKHKGTAIAELPKDYIQWLLRQDELDVYLRKALESAF-- 5M1S Chain:D ((7-243)) -------RQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAE-VADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPKTNTF---CKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRTLHGALLDAQILAEVYLAMTGGQLSLPLAMEGE-----TIQRIVRQASKLRVVFATDEEIAAHEARLDLVQKKGGSCLWRA

General information: TITO was launched using: RESULT: Template: 5M1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 760 -34071 -44.83 -156.29 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : -44.83 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.163

(partial model without unconserved sides chains): PDB file : Tito_5M1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M1S-query.scw PDB file : Tito_Scwrl_5M1S.pdb: