Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSQEPN---QSLFNQASTSIQACIECMFACKHCASACLQEEHVHMMRKCIKHCLTCVETCQLCTSLELRHS----ELAEQAMQLCANACQLCAAECSKHE--HEHCQVCAKACLACAQACQAYRA 5ARM Chain:A ((1-130)) MHVEAMISKHPQATDRSLVQCVEMCFDCAQTCAACADACLGEDKVADLRHCIRLNLDCAEICVAAGSIASRAAGTEESILRTMLQTCAEMCRMCEEECRRHAGNHEHCRICADVCKECETACRSATG

General information: TITO was launched using: RESULT: Template: 5ARM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 472 -54118 -114.66 -458.62 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -114.66 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_5ARM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ARM-query.scw PDB file : Tito_Scwrl_5ARM.pdb: