Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKTLQFGITNFEIDFKDLVMNTMIKNKRFENDVEFRGADIKDSRAVVILLHGRRQNADTIYELAEKIGFT--DITYILPLAP------ELTWYPRGFMR----DLEDNQPYLSVALERIHYIITDL-KNQNIKPENIFLMGFSQGACIVSQYLWENPQKLGGVIAFTGGLFGPELTYTSHEGLPINGTQLIFTGGKEDSWVPEQRVRDTAALFEKLGGNVTTKIYSGRDHLVSDEEVELAKTLLNI 4FTW Chain:A ((53-263)) -------------------------------LTFGRRGAAPGEATSLVVFLHGYGADGADLLGLAEPLAPHLPGTAFVAPDAPEPCRACGFGFQWFPIPWLDGSSETAAAEGMAAAARDLDAFHDERLAEEGLPPEALALVGFSQGTMMALHVAPRRAEEIAGIVGFSGRLLAPERLA----EEARSKPPVLLVHGDADPVVPFADMSLAGEALAEAGFTTYGHVMKGTGHGIAPDGLSVALAFLK-

General information: TITO was launched using: RESULT: Template: 4FTW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1085 -9795 -9.03 -49.47 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -9.03 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.608

(partial model without unconserved sides chains): PDB file : Tito_4FTW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FTW-query.scw PDB file : Tito_Scwrl_4FTW.pdb: