Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAYFEKLLDSSQQQKQLPLIFPLLIALFSGAVFSFALAPYYWWWLAILSPALLYATLHNRSAKQAFAIGWSYGFGLWFVGAFWLYTSIHVYGDTNAFLSVCMIAVMALVMGLFTAFQTWVYRRFFPETPLTFAPLWIIFEWAKTWVFTGFPWLFVGYAFTERLLDGYAPLFGIYAISFVVIVLACALVEVLRKRIFWVIPAALLVLGAWGASYIQFVQPKATKPLSVSLIQGNIPQDLKWLTEYQVRTLEIYAGLTQSEWGRDLIVWPESSIPLFQTDI--------EPFLDAMDAQAKKNHTAWVTGIPYWDVTKSHQVGSPLYYNSIMASGSD-SSGLYKKQRLVPFGE-YIPLSGLLSWVLPAMQNDISMSGFTRGESDQKPLLI-KGHALAAAICYEVAYPNLTRRN-------AEDSDFLVTVSNDAWFTGTAGPWQHLQMVQMRAKENGRWFIRAT----------NTGVTAFIDQNGHITEQAPIDKEFVLRGDLPAM-----QGQTFYNRLGDYPILGFAVLLLVLGWIYRPRKVDVSYKSRR 2E11 Chain:B ((2-264)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HDLRISLVQGSTRWHDP--AGNRDYYG-ALLEPL-AG-QSDLVILPETFTSGFSNEAIDKAEDMDGPTVAWIRTQAARLGAAITGSVQLRT--------EHGVFNRLLWATPDGALQYYDKRHLFRFGNEHLR--------------------YAAGRER--LCVEWKGWRINPQVCYDLRFPVFCRNRFDVERPGQLDFDLQLFVAN--WPS--ARAYAWKTLLRARAIENLCFVAAVNRVGVDGNQLHYAGDSAVIDFLGQPQVEIR-EQEQVVTTTISAAALAEHRARFPAMLDGDSFVL--------------------------

General information: TITO was launched using: RESULT: Template: 2E11.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1385 -23678 -17.10 -102.95 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : -17.10 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_2E11.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E11-query.scw PDB file : Tito_Scwrl_2E11.pdb: