Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIFSTNTCPVPNDLESVIHHKDEVPAELCGMSEQQVQKIWKSIESLYKTGNYPLITFCLRRQGKILLNRSIGYAQGNSPAGLQENALIATPDTPVCLFSASKMITAMLIHLLDEKGEINLLDPVSYYIPEYGVNGKRRATIFHLLSHRGGIPYVDGDVTPELLFDKDEILRRLYAARPVSPAGNHLAYHAVTAGY-ILGEIIERVTGQDLRKFVHETIEKPMDMPYFNYGLKPEYRAEVALNCATGLHPRL--GTDHYLNHVLGGGL-QLAV-DVTNDSRFMDTICPAGNIYTSAEQAGRFFEMLLSGG--SYQGKQILSEKTIFRATLPTTGVNIDRTLLIPMRYALGPMLGSNPVGLFGPMTGQAFGHLGFSNILCWADPERDISVSLLTTGKSVVGTHLPALANLLYQISIQCPRIPRDQRRSLFGSDSHETDLV 3ZYT Chain:A ((5-368)) -------------------------------VIAPGFEPVAELFGVFLEQDPDYSAQVAAYHRGVKVLDLSG--------------GPHIRPDSVTGVFSCSKGMAGLVMALLVQDGELDLEAEVVKYWPEFGVEGKSSITVAQLLSHRAGLLGVEGGLTLHEVNNSELAAAKLAELPPLWKPGTAFGYHALTI-GIFMEELCRRITGSTLQEVFEQRIRAVTGANFYLGLPE-SEESRFAQFRWAADPSWPWVDPASHFGL--AANAAVGDILDLPNIREVRAAGLSSAAGVASAEGMARIYAAALTGLEGKSAMPPLLTEETIRTVSAEQVFGI-DRVFGETGCFGTVFMKSHTRM---PFGSYRAFGHDGASASLGFADPVYELGFGYVPQTAEPGGV-GCRNFQLSSAVREVIA---------------------

General information: TITO was launched using: RESULT: Template: 3ZYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2248 -132543 -58.96 -371.27 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -58.96 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3ZYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZYT-query.scw PDB file : Tito_Scwrl_3ZYT.pdb: