Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MLLTVIYIIAITAEAMTGALSAGRRSMDWFGVVLIACVTALGGGSVR---DVLLGHYPLTWVKHPEYLVLTCCAAFVTIVIAKWMRHLHNIFLVLDALGLIGFTI------------------IGCQIALQMGHG--------------FVVSAVAGVLTGVS-GGILRDILCNDVPFVFRRELYA----SISFVAVICYWVCLDLGLSLELTVISTLVFGFTLRLIAIYF----GLEM---PKFIYKDDDAESASSTDEH------ 4TQ3 Chain:A ((15-301)) SKYLRLLRPVAWLCFLLPYAVGFGFGITPNASLQHAVLGLLSFAFWMAFSFTINALYDRDVDRLHDGLNLSMQPLVTGEISVREAWLYCIAFLALSLATAAAINEKFFLAMLGANIIGYVYSAPPRFKAWPVMDVICNALAAVLAFYAGLSIGGAEVPIAIYPAAFFLAATFYIPTAVSDYEFDKKAGLKNTPVFFGPERALKSLYPLSAITVILWAYVFLMAERIEIKVISPLIIAYTLIYTFIINSRWDGEKLNVSPNLILTPFGIISALFIAYGFAVISV

General information: TITO was launched using: RESULT: Template: 4TQ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 969 -106123 -109.52 -495.90 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -109.52 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_4TQ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TQ3-query.scw PDB file : Tito_Scwrl_4TQ3.pdb: