Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFNCDLVWMNSMSALLRFTQFVQKTFALWVIIFAALALWQPEFFVWLKAYIPWILGIIMLGMGMTMTVDD----FKGVLQSPKAVLIGVVAQFVVMPGLAFILCKLFNLPPEIAVGVILVGCCPGGTASNVITYMAKGNV----ALSVACTSVSTLLAPVLTPAIFYLLASQWLKIDAASMFISILQVVLLPIVIGLILRTWLKRQVESYIQVMPLVSVIAIVAIVAAIIGGSKAAILQSGLLILAVVILHNGLGYLLGFAAARFFKLPYADSKAIAVEVGMQNSGLGVALAAVHFAASPITAVPSAIFSLWHNISGPALATYWASKHKQE 1ZCD Chain:A ((59-171)) ----------------------------------------------MLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAA--FPVIAAIGGMIVPALLYLAFNY-A-DPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALAIIDDLGAIIII-------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZCD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 225 -34881 -155.03 -332.20 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -155.03 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_1ZCD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZCD-query.scw PDB file : Tito_Scwrl_1ZCD.pdb: