Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQTPEALTTEQFKQAIIDKG-QYYH--I-YHPFHVMMYEGKATQQQIQAWVANRYYYQINIPLKDAAIMANCPD--QRVRQEWIQRMIDQDGEYPDGGGREAWLRLAEAVGLSREQVISEELVLPGVRFAVDAYVNFARRASWREAASSSL-TELFAPQIHQSRLESWPQHYPWIDDKGYEYFRSRLSQARRDVEHGLTITLDSFTTHEQQQRMLEILQFKLDILWSILDALTLAYVHNEAPYHSVTQERVWHKGLFK 3DDE Chain:A ((4-226)) ----------IIDLTKLEQKVATMWDSILTNSPFIHEVLDGKATKALYAIYMTETYHYTKHNAKNQALVGIMGKDLPGKYLSFCFHHAHEEA------GHELMALSDIASIGFDREDVLSS-KPLPATETLIAYLYWISATGNPVQRLGYSYWAENVYGYI-DPVLKAIQSTLD-LTPQSMKFFIAHSKIDAKHAEEVNEMLHEVCKTQEDVDSVVAVMENSLVLTARILDDVWKEYQLFQS-----------------

General information: TITO was launched using: RESULT: Template: 3DDE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 934 -21054 -22.54 -97.47 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -22.54 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_3DDE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DDE-query.scw PDB file : Tito_Scwrl_3DDE.pdb: