Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFLQRSNQHSNNNRLKRMKSHLRVHYFQHIAGEGFGSCYSFLKAHHATITATEFFALPVDLPLEIEALPYIEDVDLLLIMGGTMSVNDEANYPWLKIEKRWIRRYLAAGKPAIGLCLGGQLIANALGAAVSRNRYQELGWSTVQRVPNL-------------------------PKESFL--LPE-KINVMQWHSETF-EIPKGAIHLAENSVCRNQMYQIG-SNVLGFQFHPEMTP-----KVLNLLLENEQELSIFKGEYVQSLDELHHCDIQKFEQGNQLLNRAIEFVVNQ 3UOW Chain:A ((7-255)) ----------------------YDKILVLNFGS-QYFHLIVKRLNNIK--IFSETK--DYG---VELKDI-KDMNIKGVILSGGPYSVTE-AGSPHLK--KEVFEYFLEKKIPIFGICYGMQEIAVQMNGEVKKSKTSEYGCTDVNILRNDNINNITYCRNFSSAMDLYSNYKLMN--CCLFENIKSDITTVWMNHNDEVTKIPENFYLVSSSENCLICSIYNKEYNIYGVQYHPEVYESLDGELMFYNFAY--------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 800 -94680 -118.35 -523.09 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -118.35 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_3UOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UOW-query.scw PDB file : Tito_Scwrl_3UOW.pdb: