Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPAIRRTVTFPEISMERLKSILGAYNGHLKQIEQRLDVKITHRGDVFYIDGEIDAVGRAEALLQRLYEETEASQQISADLLHLLIQSSQTERNFELVGEEMDEHDAPMDVYFQTRKGRINPRGANQKRYVQRI-LQSDISFGVGPAGTGKTYLAVAAAVDMLERNEIQRILLVRPAVEAGEKLGFLPGDLT---------QKIDPYLRPLYD-ALY---EMLGFEKVAKLIERQVIEVAPLAYM---RGRTLNHSFVILDEAQNTTPEQMKMFLTRLGFGSRAVITGDITQVDLPRGQQSG------LA-HALRVLENIKEIHITRFHSRDVVRHQLVQKIVEAYEAWDGEQQRLNAEARAERKARQEALIAENDTAADLQHQDA 5EAN Chain:A ((625-833)) ---------------------------------------------------------------------------------------------------------------------NILKGLNKPQRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSAC--GFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLF---FSRRFVLVGDHQQ--LPPLVVNREARALGMSESLFKRLER---NESAVVQLTVQYRM--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EAN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 889 -49492 -55.67 -267.52 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -55.67 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_5EAN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EAN-query.scw PDB file : Tito_Scwrl_5EAN.pdb: