Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSQPLLDCWNLDQILDDLNAHGFAIVNQAYS-AEYHTQVAKECSHHFDEFREAGIQNGVVSTIRSDHILWINESLPVAEQHVETL-TSFCQHLNQAFFLGIKE-VEAHFACYNPGEFYALHRDNPQQKNDRIMSTVYYLHPQWQDDWGGQLRLQDKNDI------WHIITPEPNRLVIFQSN--LLHEVLVSK---QQRLSITAWLRSGNSIWV 4NHY Chain:D ((79-321)) -------------------DPFLHCVIPNFIQSQDFLEGLQKELMNL--DFHEKYNDL--YKFQQSDDLKK--RREPHISTLRKILFEDFRSWLSDISKIDLESTIDMSCAKYEFTDALLCHDDEL---EGRRIAFILYLVPPWDRSMGGTLDLYSIDEHFQPKQIVKSLIPSWNKLVFFEVSPVSFHQVSEVLSEEKSRLSISGWFHGPSL---

General information: TITO was launched using: RESULT: Template: 4NHY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 706 -9079 -12.86 -53.40 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -12.86 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4NHY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NHY-query.scw PDB file : Tito_Scwrl_4NHY.pdb: