Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNIYQFEAELLVGDIKQFADYKGKVLLIVNTASKCGFTP-QFAGLEKLYEKYKDLGLEVLGFPCNQFGGQDPGSNKEIGTFCQRN-YGVKFPMFAKVDVKGPEAHVIFRYLTREAKGILGSSTIKWNFTKFLVGKDGSVLNRYAPTTKPEALEADIEKALAS 2P5R Chain:B ((10-168)) -ESVHDFTVKDAKENDVDLSIFKGKVLLIVNVASKCGMTNSNYAEMNQLYEKYKDQGLEILAFPCNQFGEEEPGTNDQITDF-VCTRFKSEFPIFDKIDVNGENASPLYRFLKLGKWGIFGD-DIQWNFAKFLVNKDGQVVDRYYPTTSPLSLERDIKQLLE-

General information: TITO was launched using: RESULT: Template: 2P5R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 719 -13816 -19.22 -88.00 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -19.22 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2P5R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P5R-query.scw PDB file : Tito_Scwrl_2P5R.pdb: