Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIREILLADTHNYYGILDERFIDLAHQFSRLQDARTGQGGAALAVYFRGQKVVDIYTGLKSQ--TEAWQPDTLAVCYSTGKGVLATLAHILVSEGFLEYDKPIATYWPEFAQNGKEQMTLRHVLSHQSGMFDVRNIIESA--REMLDWSHMLVVVAATKPRFLAGEGNAYQALTFGWLVGGVLEKATGQSLDQLMQNYLVEPLQLDGAYFGTPAN-ELDRVARLIIQPKPEKPASTQVEKPKKPQTRK-SSLSEKVITWTGQDPQDFQDAMIPKGMKNFSFFSDEGLQAVIPAANGTFTANSLAKIYAMLANQGEWDGQQLIRPEVFKELSTIQSYARDRVMPIPMNWRLGYHRIITMGKRAKNGFGHIGYN-G--SGAWCDPERDLSFAYTHNLQIGSITGDYRLWGLTQEALRCTDQILKGRKGWF 2DRW Chain:B ((7-360)) ----------------------AIQGILDDHVAR--GVVGVSLALCLPGEETSLYQSGYADKFNKMPMTGDHLFRIASCTKSFIATGLHLLVQDGTVDLDEPITRWFPDLPK--AAQMPVRILLNHRSGLPDFE-TSMPMISDKSWTAQEIVDFSFRHGVQKEPWHGMEYSNTGY-VLAGMIIAHETGKPYSDHLRSRIFAPLGMKDTWVGTHETFPIEREARGYMH--AAADDE----NP---QWDVSGAGDPVD----------------GVWD----STEWFPLSGANAAGDMVSTPRDIVKFLNALFDG------RILDQKRLWEMKDNIKPAFF-PGSNTVANGHGLLLMRY---GSSELKGHLGQIPGHTSIMGRDEETGAALMLIQNSGAGDFE-SFYLKGVNEPVDRVLEAIKN------

General information: TITO was launched using: RESULT: Template: 2DRW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2236 -6179 -2.76 -17.91 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -2.76 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_2DRW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DRW-query.scw PDB file : Tito_Scwrl_2DRW.pdb: