Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTEVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSR--NC-DEAIEEIRKIPHLHNVNFFK 1ZPV Chain:C ((6-74)) ------------------------------------------------------------------------------------------------------------------------------------------------------AIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVL--DEYFTMMAVVSSDEKQDFTYLRNEFEAFGQTLN-----

General information: TITO was launched using: RESULT: Template: 1ZPV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 201 -44818 -222.98 -679.06 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain C : 0.56 3D Compatibility (PKB) : -222.98 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.693

(partial model without unconserved sides chains): PDB file : Tito_1ZPV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZPV-query.scw PDB file : Tito_Scwrl_1ZPV.pdb: