Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVNFFHEKEKIMRYDFGKVYKEIRESKGLTQEEVCG--GVLSRTSLSKIESGKTTPKYENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQDYLKT---HHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDL-----KILNTILFSFP---IEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTLLEDAKNKKSYDRLAICYVRIGICT--------DDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQAKER 5FD4 Chain:A ((21-324)) ------MEVWFMNDKEFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGASKPTFSKIQYIATRLGMGLYELMPDYVSLP-ERYSKLKFDVLRTPTYGNEDLAEKRDAMMTEIYDDYYDELPEEEKIAIDAIQSRIDTLES---GTAGFGKEILEDYFEQIFRKRKYELNDLLIVRLHLEYVRLSSCDSEIFRQFLKIIEHLHEQINIIN--SNDLFVLRDTLLSCVNILGSKKYYEPIPKIFDSVDKIIQSTQDFQKKPIVSVLKWKYALFVDKDRDEAEKHYLDAVLFAKLIENRELEQKIEEDWRVDNQ----

General information: TITO was launched using: RESULT: Template: 5FD4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1129 20098 17.80 71.02 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 17.80 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_5FD4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FD4-query.scw PDB file : Tito_Scwrl_5FD4.pdb: