Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKKEKKMAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWSPDHTKMGKGITLSNEEFQTMVDAFKGN 5J0K Chain:A ((6-79)) ---TRTEIIRELERSLREQE-ELAKRLKELLRELERLQREGSSDE-DVRELLREIKELVEEIEKLAREQKYLVEELKRQ

General information: TITO was launched using: RESULT: Template: 5J0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 119 -1042 -8.75 -14.07 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -8.75 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_5J0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J0K-query.scw PDB file : Tito_Scwrl_5J0K.pdb: