Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEKTRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGM-DGQSSPVPENIVLPETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ 2EV9 Chain:B ((5-245)) ----------AVLGHPVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEVRR-AFRGVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTDAPGFLEALKAGGIPLKGPALVLGAGGAGRAVAFALREAGLEVWVWNRTPQRALA------LAEEFGLRA--VPLE-----KAR--EARLLVNATRVGLEDPSASPLPAEL-FPEEGAAVDLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWTG-----------------

General information: TITO was launched using: RESULT: Template: 2EV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1381 -124227 -89.95 -517.61 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -89.95 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_2EV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EV9-query.scw PDB file : Tito_Scwrl_2EV9.pdb: