Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQFLDNIKDLEVTTVVRAQEAL------------DKKETATFFIGRKTCPYCRKFAGTLSGVVAETK--AHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDSSMSAQEIKDFAGL 2L5L Chain:A ((3-122)) --LATEGNGKVIHLTKAEFLAKVYNFEKNPEEWKYEGDKPAIVDFYADWCGPCKMVAPILDELAKEYDGQIVIYKVDTEK------EQELAGAFGIRSIPSILFIPMEGKPEMAQGAMPKASFKKAID-

General information: TITO was launched using: RESULT: Template: 2L5L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 444 1521 3.42 14.34 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 3.42 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2L5L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L5L-query.scw PDB file : Tito_Scwrl_2L5L.pdb: