Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLKFTEDAWADYC------YWQNQDKKTLKRINKLIKDIQR-----DPFTGIGKPEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY 5IWH Chain:A ((2-88)) GIKSFKHKGLKLLFEKGVTSGVPAQ---DVDRINDRLQAIDTATEIGELNRQIYKLHPLKGDREGYWSITVRANWRITFQFINGDAYILN-----

General information: TITO was launched using: RESULT: Template: 5IWH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 242 1302 5.38 17.13 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 5.38 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_5IWH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IWH-query.scw PDB file : Tito_Scwrl_5IWH.pdb: