Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQKYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQPITRIEIDAIRGVN---SSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHLEELPVIDELEIQAQESQLFGERIEEDENQ 4EM0 Chain:B ((48-132)) ---------------------------------------------------------------------PYLKLTEPILE---KHNIYYGQWLILRDIAKHQPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSL-EDKRQKFLTLTPKGHELY----------------------------------

General information: TITO was launched using: RESULT: Template: 4EM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 280 -12014 -42.91 -146.51 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -42.91 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_4EM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EM0-query.scw PDB file : Tito_Scwrl_4EM0.pdb: