Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------MEQLHFITKL--------LDIKDPNIQIL-------DIINKD-THKEIIAKLDYDAP------SCPECG---------SQMKKYDFQKPS---KIPYLETTGM-PTRILLRKRRF-----KCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLS-------ISTSTVIRKLNDFHFKHDF-SCLPEIMSWD--------EYAFTKGKMSFIAQDFNNLNIITVLEGRTQAVIRDHFLKYDRAVRCR--VKIITMDMFSP---YYGLA------------------KQLRFHIVQHLSRA-MSRVRVQIMNQFHRKSHEYKAIKR----------YW----------KLIQQDSRKLSDK--QFYRPTFR--------------------MHLTNKEILNKLLSYSED-------LKHHYQLYQLLLFHFQNKE----------PEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNA----------------KPEATNNLIKLIKRN------------AFGFRNFENFKKRIFIALNIKKERTKFVLSRA-- 5L8S Chain:A ((1-604)) MINFPKPTVEQFFRTYTITNFAVSSDEKRLVFNANLNGKMNLWAMDLPDTYPYLFAHRDESCNFIKFDPENRYVLAGFDKDGDENYQIYAIPNEGGLPHPLITGDASEKYYFSHLSADGKCVYYETSKENPSFLNTRIRNLETGEDRLLNVGEVSTTELAAVSENEESFVYLRAFANTYIVGFVKMGEETFNITPDPEKVHVAMEPVFTDNETIYFATDYDSDEMYLAKFDLTSKEFSKVLAFDGESIQSVKWDKDNKAFYLITVKGVTDILYRYDVATDKVEECSLPVDIIEQIQVAKSGNLYILGRSATVPHNVYQSSNGVEWKQLTNNRVLGLSPEDMVEPDIVSYTSFDGMEIEALLFKAKPENDNGYTIFWPHGGPQSAERKMFRSMFQCFINRGYTIFAPNFRGSTGYGSAFTKLVELDWGEGPRLDCIAGIEWLFESGFTDRNKLFLVGGSYGGYMALLLHGRHSDYFRAVVDIFGPSDLFTFINSVPPHWKPIMERWLGDPERDKERFIKDSPVTYLDGMVKPMLVIQGAKDPRVVKEESDQIVAKLKEKGRDVEYLVLEDEGHGFSKKENEIKVYSLMLAFLEKHQALEHHHHHH

General information: TITO was launched using: RESULT: Template: 5L8S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2220 23184 10.44 56.68 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 10.44 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.151

(partial model without unconserved sides chains): PDB file : Tito_5L8S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L8S-query.scw PDB file : Tito_Scwrl_5L8S.pdb: