Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHFTFLGTSSGVPTLSRNVSGLAVR----NSKNKDWILVDAGEGTQHR---IQQAR-------------------------------------------------------LSLQNLIAICITHVHGDHCYGLVGLLASAGMNARNNPLIVIAPKEIQQWFEITA-QLTDL---H--LPYSIKFIDVNEATRPQ--QLTDELFIQAHPLSHRVP--------SFAFSIYIKSTQKKIDIQALTQLGIPKGDIWGHLKRGYDVEFEGRILKSQDFIKIQNQQIHAIIGGDNDRPE-----LL-----ADAC-----KDAQLLIHEATYLQTVLDKVGKGPMHSSAKMVAEFAEQ----------QSLDNLILTHFSPRHQDKTGQQAIIEEVRQF-----YKG-HFYLANDFDEFTLNETGQLLKIE 4OJV Chain:A ((3-369)) VFEITILGANGGP-T-EYGTQCFILKPARTE--DPELIAVDGGAGMYQLREMLVQG-DDELVPSFYEHDREPIEFFIDSKLNIQKGLSKSLLQSLKRQGEHFESANTMKKTYEVFQGITDYYITHPHLDHISGLVVNSPSIYEQENSKKKTIWGLPHTIDVLQKHVFNDLIWPDLTAERSRKLKLKCLNP-K-EVQKCTIFPWDVIPFKVHHGIGVKTGAPVYSTFYIFRDRK-----------------------------------------------SKDCIIVCGDVEQDRRESEESLLEEFWSYVAENIPLVHLKGILVECSCPLSSKP-EQL-YGHLSPIYLINELSNLNTLYNSSKGLSGLNVIVTHVKSTPAKRDPRLTILEELRFLAEERNLGDLRISIALEGHTLFL----------

General information: TITO was launched using: RESULT: Template: 4OJV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1213 28349 23.37 112.50 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 23.37 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_4OJV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OJV-query.scw PDB file : Tito_Scwrl_4OJV.pdb: