Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRIETEASAHKHRVPLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWGVPTRNQAK--YNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGWSLGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFKLTAPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDIDCSVLAVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPS-GHMGVVSGSQAPTSVWPEMSNWLAMRSD 3E0X Chain:A ((1-245)) ------------------------------------------------------------SNAMLHYVHVGN----KKSPNTLLFVHGSGCNLKIF-----GELEKYL--EDYNCILLDLKGHGESKGQCPSTVYGYIDNVA-NFITNSEVTKHQKNITLIGYSMGGAIVLGVALKKL-PNVRKVVSLSGGARFDKLDKD--F----------MEKI----Y-----H--NQLDNN-------------YLLECIGGIDNPLSEKYFE------TLEKDP--------DIMINDLIA--------CKLIDLVDNLKNIDIPVKAIVAKDELLTLVEYSEIIKKEVE--NSELKIFETGKHFLLVV---NAKGVAEEIKNFI-----

General information: TITO was launched using: RESULT: Template: 3E0X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1461 -57653 -39.46 -238.23 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -39.46 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_3E0X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E0X-query.scw PDB file : Tito_Scwrl_3E0X.pdb: