Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTLAERLRYAMEVLPPKKIKGV-------ELARAVGVKPPSVSDWLSGKSKTMEGENLLRASKFLNVNPSWLASGTGEIQTSTKDKFKQLDIEEFKKKY---------------NISDSDEALLFSTIIEKPFI-----PSSKRWVPVKAYSKMGM-DGYFTDIGYEGNAGDGYVPTHTAGSRAYAV-KGTGDSMFPAIRNGWYVVCDPDADLVPNEFVQVCLKDGRCTIKEFVGIN----------GGVLSLLSVNGGERFFF--EMDEVESITAITDIVPPSQHRQEHPYSH 4QFR Chain:C ((28-322)) YTTFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVG--MLTITDFINILHRYYKSALVQI-----YELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRVLKCYLHETLEAIINRLVEAEVHRLVVVDEHDVVKGIVSLSDILQALV---------

General information: TITO was launched using: RESULT: Template: 4QFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 982 4590 4.67 19.29 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : 4.67 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.208

(partial model without unconserved sides chains): PDB file : Tito_4QFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QFR-query.scw PDB file : Tito_Scwrl_4QFR.pdb: