Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLPILSFPDPRLRTIAKPVEEVTDEIRQLAADMFETMYAAPGIGLAASQVDRHIQLIVMDLSESKDEPMVFINPKVTPLTEETQPYEEGCLSVPQIYDKVDRPSRVKIEAINLEGQAFEIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRVREKVEKIVRQREREKVAVKR 1G2A Chain:C ((1-164)) -SVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGET-GIEEGCLSIPEQRALVPRAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVEKLDRL----------

General information: TITO was launched using: RESULT: Template: 1G2A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 811 -89775 -110.70 -547.41 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.87 3D Compatibility (PKB) : -110.70 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.664

(partial model without unconserved sides chains): PDB file : Tito_1G2A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G2A-query.scw PDB file : Tito_Scwrl_1G2A.pdb: