Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKFNNNQSDNLFSQVFGVAKKLSSTGLNILQQSQIGEVSKLVEPLSNGKTVEGSARNKSPFEVEQYESPLQMLREHL----PKVTRQVFGRHFRKVNGIATFISPDWNEKISSYLFDWLNDFSSKSTLTEKILEEAGAKDLFELTKDTSRSQRLSQALIEQNKLIASIQGAITGVSGMVGAAVDIPVSLVLVLRTIYQTGRSHGFDLTEATDQDVVEFIFKEVDISLIAEKQTLLLALKALRNMLETQDIQQFQQVLGSSNDIETLKSWLVDENGKFKWNWLNKVPQLAVVGKFTPVAGAVLSAVYSWKLQEDVGHKAQAIFGAARHYLNEHPNEHLSPLQAYYAAVTLIQKASPRLLNVGENGSVHATQHHKIENHDVISKVSVVVKSNTSEKSEEKVQENVHQGIEHLAEKHVVEHEHSEQKPALEPESEENDDVIEGQKYS----- 3FGA Chain:B ((1-403)) ----PPADQEKLFIQKLRQC----CVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLQLLELFD--SEDPRERDFL---KTTLHRIYGKFLGLRAYIRKQIN-----NIFYRFIYETEHHNG--IAELLE--ILGSIINGFALPLKEEHKIFLLK--VLLPLHKVKSLSVY------HPQLAYCVVQFLEKDSTLTEPVVMALLK------YWPKTHSPKEVMFLNELEEILDVIEPSEFVKIMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDN-----------AAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKLKEKLKMKEREEAWVKIENL

General information: TITO was launched using: RESULT: Template: 3FGA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1481 -58681 -39.62 -148.94 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -39.62 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_3FGA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FGA-query.scw PDB file : Tito_Scwrl_3FGA.pdb: