Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIKSDRWIREMSE-KHGMIEP-YAENQVRFDKNGEKLISYGVSSYGYDVRCAREFKVFTNVH-----SAIVDPKNFDEKSFIDIESDVCIIPPNSFALARTIEYFRIPRNVLTVCLGKSTYARCGIIVNV--------TPLEPEWEGHVTLEFSNTTNLPARIYAGEGVAQMLFFESDEVCETSYKDRGGKYQGQTGVTLPKT 2R9Q Chain:B ((10-176)) TGILADGAIRALFAGDKLKSEADLDVDQVQP--------------ASLDLRLGSKAYRVRASFMPGPGTRVIDKLNRF-LHEVDL-SQGAVLETGCVYIVPLMESLALPADMSASANPKSSTGRLDIFTRVMTDNAQEFDKIPAGYTGPLYLEISP-RTFPIVVRRGSRLSQIRFRIGHA------------------------

General information: TITO was launched using: RESULT: Template: 2R9Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 655 -13781 -21.04 -93.11 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -21.04 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_2R9Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R9Q-query.scw PDB file : Tito_Scwrl_2R9Q.pdb: