Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVIALFSKNWSRTMSATIVNTTDDNFQADVLDAETPVLVDFWAGWCAPCKAIAPVLEDLSSEYAGKVKIVKVDVTSCEETAVKYNIRNIPALLLFKNGEVVAQQIGAVPRSKLVSFIDENV 2BTO Chain:T ((4-106)) -----------------IIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDAN-

General information: TITO was launched using: RESULT: Template: 2BTO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 427 -81255 -190.29 -788.88 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain T : 0.81 3D Compatibility (PKB) : -190.29 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.770

(partial model without unconserved sides chains): PDB file : Tito_2BTO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BTO-query.scw PDB file : Tito_Scwrl_2BTO.pdb: