Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------MNRTLKELILAAEFAAIISVLSQMTIPFGLV-------------PLTGQTFAIGLTATFLGRKLGLISVGVYLLLGLI-----GLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI---ERTDAHY-LYAIFANLMGSLAALICGTI-----------------------WLKISGDLTFT----TAFASGLLPFLLPEAIKATGAAYIGIL------MKKRLNRFLHTN 4ECF Chain:A ((2-261)) AGESITAVGSTALQPLVEAAGEQYTGEHLGTFINVQGGGTGTGLSQIQEGAVQIGNSDLFAGEQKGINARQLVDHRVAVVGITPIVNKKVGVKNLSTNQLIKIFTGQITNWKEVGGADQSIVLINRAQGSGTRATFEQFGLANHRSKTAQEQDSSGMVRSIVATTPGAISYVAFSYVNKTVQALSLNHVAPTEVNVTTNDWRIWSYEHLYTKGHPTGLTKAFITYVQSPAIQNTLVRQLGYLSPDQMLVERDANGHITKT

General information: TITO was launched using: RESULT: Template: 4ECF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 897 -59655 -66.50 -333.27 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -66.50 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_4ECF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ECF-query.scw PDB file : Tito_Scwrl_4ECF.pdb: