Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKIKIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALGNHGKRRPLRFDSSSRIQ 2H5X Chain:B ((1-114)) MIASVRGEVLEVALDHVVIEAAGVGYRVNATPATLATLRQGTEARLITAMIVREDSMTLYGFPDGETRDLFLTLLSVSGVGPRLAMAALAVHDAPALRQVLAD-

General information: TITO was launched using: RESULT: Template: 2H5X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 435 -72129 -165.81 -700.28 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -165.81 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_2H5X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H5X-query.scw PDB file : Tito_Scwrl_2H5X.pdb: