Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYDVALRLGE-------------MKEI--RTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHA---------------------------VGGTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHL--TEIQPTDTVMILGASGAVGSSLIQLLHEKGIRILTSASS-KNEEKVKKLGASAFAAYDKTN-----------------PGLQFA-----DQADLVIDATKGSIKGETGIQIMKPGGRYVALNDLPDLD--LRQ---KKEGFYE-SFVPRKEYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEG-HPPAGKIILSFEK 3KRT Chain:D ((67-420)) ------------------------ELGPGEALVAVMASSVNYNSVHTSIFEPLSTFGFLERYGRVSDLAKRHDLPYHVIGSDLAGVVLRTGPGVNAWQAGDEVVAHCLSVELESSDGHNDTMLDPEQRIWGFETNFGGLAEIALVKSNQLMPKPDHLSWEEAAAPGLVNSTAYRQLVSRNGAGMKQGDNVLIWGASGGLGSYATQFALAGGANPICVVSSPQKAEICRAMGAEAIIDRNAEGYRFWKDENTQDPKEWKRFGKRIRELTGGEDIDIVFEHPGRET-FGASVFVTRKGGTITTCASTSGYMHEYDNRYLWMSLKRIIGS-H---FANYREAWEANRLIAKGRIHPTLSKVY--SLEDTGQAAYDVHRNL-HQGKVGVL---

General information: TITO was launched using: RESULT: Template: 3KRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1506 -561 -0.37 -2.00 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : -0.37 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3KRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KRT-query.scw PDB file : Tito_Scwrl_3KRT.pdb: