Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFLKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIH-PDMYHIYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKI-PPSLRNIYKELYDDLGIAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIVFILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPIDWQLPETV 4WS6 Chain:A ((17-238)) ---LSELVERGWAAAL--EPVADQVAHMGQFLRAEIAAGRRYLPAGSNVLRAFTF-PFDNVRVLIVGQDPYPTPGHAVGLSFSVAPDVRPWPRSLANIFDEYTADLGYPLPSNGDLTPWAQRGVLLLNRVLTVRPSNPASHRGKGWEAVTECAIRALAARAAPLVAILWGRDASTLKPMLAAGNCVAIESPHPSPLSASRGFFGSRPFSRANELLVGMGAEPIDWRLP---

General information: TITO was launched using: RESULT: Template: 4WS6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1146 -2586 -2.26 -11.75 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -2.26 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_4WS6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WS6-query.scw PDB file : Tito_Scwrl_4WS6.pdb: