Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNRANEDIITLGANAASKIVT--EKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARSFEVKEACYGGTAALHMAKEYVKNH--PERKVLVIASDIARYGLASGGEVTQGVGAVAMMITQNPRILSIEDDS-VFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKELS----------GRGLEDYQAIAFHIPYTKMGKKALQSVLDQT-----------------DEENQERLMARYEESIRYSRRIGNLYTGSLYLGLTSLLENSKS-LQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEMLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI 5HWO Chain:A ((5-417)) KRVGIEALAVAVPSRYVDIEDLARARGVDPAKYTAGLGAREMAVTDPGEDTVALAATAAARLIRQQDVDPSRIGMLVVGTETGIDHSKPVASHVQGLLKLPRTMRTYDTQHACYGGTAGLMAAVEWIASGAGAGKVAVVVCSDIARYGLNTAGEPTQGGGAVALLVSEQPDLLAMDVGLNGVCSMDVYDFWRPVGRREALVDGHYSITCYLEALSGAYRGWREKALAAGLVRWSDALPGEQLARIAYHVPFCKMARKAHTQLRLCDLEDAADAAASTPESREAQAKSAASYDAQVATSLGLNSRIGNVYTASLYLALAGLLQHE-AGALAGQRIGLLSYGSGCAAEFYSGTVGEKAAERMAKADLEAVLARRERVSIEEYERLMKLPADAP--EAVAPSPGAFRLTEIRDHRRQYA-

General information: TITO was launched using: RESULT: Template: 5HWO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2430 1457 0.60 3.83 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 0.60 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_5HWO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HWO-query.scw PDB file : Tito_Scwrl_5HWO.pdb: