Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIKEVRKSIARIKTVLREQAK 5GAF Chain:Z ((2-62)) -KAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAG

General information: TITO was launched using: RESULT: Template: 5GAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Z - contact count / total energy / energy per contact / energy per residue : 118 -8010 -67.88 -131.31 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain Z : 0.81 3D Compatibility (PKB) : -67.88 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.832

(partial model without unconserved sides chains): PDB file : Tito_5GAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAF-query.scw PDB file : Tito_Scwrl_5GAF.pdb: