Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAISTKELMEVTLGKKLGAGSFGSVFVGV-LPSGNFVAVKILELSDDAPSN---TEVEIHRKMVHPNIIRYLYSRIDLESTPKKLYVYLEFVTGGSVTSLMKSLPNGCLPYAVVRVYARHMFQGLEYLHSNQVAHRDIKGDNVLISMDTGTAKLADFDQAKIMNTHGTLRKAATATLAGTPYWMAPEVITDEDGYDPFKADIWSAGCTVAEMITGRAPWTPMPNVMHIMNKLALSTGWPDAVPKDAKELGSQDAYDFLDLCFQRDVSKRPPAATLLKHVFLRV 4ZY6 Chain:A ((27-276)) ---------------EKIGQGASGTVYTAMDVATGQEVAIK------QPKKELIINEILVMRENKNPNIVNYLDSYL----V--ELWVVMEYLAGGSLTDVVTET---CMDEGQIAAVCRECLQALEFLHSNQVIHRNIKSDNILLGMD-GSVKLTDFG----FCAQITPEQSKRSTMVGTPYWMAPEVVT-RKAYGP-KVDIWSLGIMAIEMIEGEPPYLN-ENPLRALYLIA-TNGTPEL--QNPEKL-SAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKI

General information: TITO was launched using: RESULT: Template: 4ZY6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1114 -31206 -28.01 -131.12 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -28.01 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4ZY6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZY6-query.scw PDB file : Tito_Scwrl_4ZY6.pdb: