Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKDGIIVDIDAAATAIKSAISQAEEKAGISIKS-VNVGLPGNLLQVEPTQGMIPVTSDTKEITDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYTGPRTI---LHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVATIRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEVIGEVEAVEVTEAYLSEIISARIKHILEQIKQ---ELDRRRLLDLPGGIVLIGGNAILPGMVELAQEVFG--VRVKLY-------VPNQVGIRN-----PAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSHQPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGLIGSMFDE 1E4F Chain:T ((7-390)) ---TVFYTSIDIGSRYIKGLVLGKRDQEWEALAFSSVKSRGLDEGEIKDAIAFKESVNTLLKELEEQLQKSLRSDFVISFSSVSFEREDTVIERDFGEEKRSITLDILSEMQSEALEKLKENGKTPLHIFSKRYLLDDERIVFNPLDMKASKIAIEYTSIVVPLKVYEMFYNFLQDTVKSPFQLKSSLVS---TAEGVLTTPEKDRGVVVVNLGYNFTGLIAYKNGVPIKISYVPVGMKHVIKDVSAVLDTSFEESERLIITHGNAVYNDLKEEEIQYRGLDGNTIKTTTAKKLSVIIHARLREIMSKSKKFFREVEAK--IGIPGGVVLTGGGAKIPRINELATEVFKSPVRTGCYANSDRPSIINADEVANDPSFAAAFGNVFA--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1E4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 1905 -54221 -28.46 -151.88 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain T : 0.69 3D Compatibility (PKB) : -28.46 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_1E4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E4F-query.scw PDB file : Tito_Scwrl_1E4F.pdb: