Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLTVTEITVSSACKCLGVSTSGYYAWRKRQI-----------------YVNQKYTDLKAVYWQHYARLGAPSLVHDMHDLGY-----CMSERTVGRMLKKISLRSKIARK 2K27 Chain:A ((38-142)) ---AHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQ-NPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIRTKVQQPFNL--

General information: TITO was launched using: RESULT: Template: 2K27.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 124 -8063 -65.02 -97.14 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -65.02 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_2K27.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K27-query.scw PDB file : Tito_Scwrl_2K27.pdb: