Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQKQPYTPGEFQWSFLLPKYWGVWIAITFLMLLAILPWAIQWRLAHGLANLAWKYL-KSRRKTTIRNLEVCFPEWSPEKVQQQAKQVFVDMMLGIFETLNAWYK-PY-WFKNRVTIEGLEHITNAQAQGKGVLLLGTHSTLLDAGGYVCAQYFEPDVVYRPQNNPLLDMLIYRCRGTIYKAQIDH--DDMRGLIRHLKEGDAIWYSPDQDFGLKQGVMAPFFGVPAATVTAHRRLLKISKAVAVPLYFYRHGNVQNPKYHILIEPAVDNLPSEDEVDDATRVNKIIENQLRIAPTQYMWFHRRFKTRPEGYEEIY 5KNK Chain:B ((15-297)) -------------------------MNYQLLKTFSRQPIQFGRFLARLLAGLVNTLKITRTSKSIELNLRIALPYLTPQQRIAITEKAVRNELTSYFEFLSIWGSSNSKNISRIHRIEGEHFFHEALAAKKGVVLIVPHFGTWAVMNAWCAQFTSMTILYKPVKNADADRFVREARSREQANLVPTDESGVRQIFKALKQGETTVILPDHTPN-VGGDMVNYFGVPLASSNLSAKLIQKTKAKALFLYAIR--NEN-DGFTMHIEPMDEKIYEGTADDGTYVIHQAIEQLIYQYPEHYHWSYKRFKANPALD----

General information: TITO was launched using: RESULT: Template: 5KNK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1197 9654 8.06 34.72 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 8.06 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_5KNK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KNK-query.scw PDB file : Tito_Scwrl_5KNK.pdb: