Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIMQIRQFQAQRMQAPRDFTPIENT------PICVEIGAGKGKHALLFSGQNPQHTLYAIERTREKFL-AMQKQHGLEPRD-NLIPVHADALPWVVHALYPAQVEHFFILYPNPEPHNPAQRWLNMPFFEFLLSRLKTGGTITLAGNIPEYIEEAEQQLLNVWKLPYQKEKIAQTSARTHFEIKYLERGELCQQLIMTKPEGYNTRFDEFAPLQGQIHVE 2FCA Chain:A ((21-121)) ------------ISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQKVKDSEAQNVKLLNIDADTLTDVFE---PGEVKRVYLNFSDPWP--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FCA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 396 18162 45.86 195.29 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 45.86 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_2FCA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FCA-query.scw PDB file : Tito_Scwrl_2FCA.pdb: