Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQRCLMDSKTTKLPAYSETLSIEGMTCASCVGRVEKALKKVENVEIANVNLATEKAVVYSNQPIQREALVKAVERAGYDVPKAAPVELSIE--GMTCASCVARVEKALKKVEGVQNTTVNLATEQAWVQADPS-VNVEDLIRAVKKAGYDAKASEKNQDEQ--LDKKASELDQLKKDLIISIVLALPVFILEMCSHLIPAFHMWVMHTIGQYNSWLLQFVLTTLVLVFPGRRFYQKGIPALWRLAPDMNSLVAVGTLAAYSFSVVATFMPQVLPEGTVNVYFEAAAVIVSLILLGRYFEAKAKGRTSQAIQHLVGMQPKTARIQRNGQIVEVAVAEVVSGTIVEIRPGERVPVDGEVVEGHSYIDESMITGEPVPVEKIIGQQVVGGTVNQNGTLNIRATAVGSSSVLSQIIRMVEQAQGSKLPIQGLVDKVTMWFVPAVMLIAAITFLVWFIWGPEPALTFGLVNAVAVLIIACPCAMGLATPTSIMVGTGRGAELGVLFRKGEALQLLQEAQVVAVDKTGTLTEGKPTLTDFNVQSGFERNQVLTLVASVEAKSEHPIALAIVQAAESEGLNLLPVTAFNSITGSGIEAEVSGQKVQIGADRYMHELRLDTSSFQAIA-AQLGEEGKTPLYVAIDQQLAAIIAVADPIKETTYAAIEALHQLGLKVAMITGDNRHTAQAIAKKLNIDEVVAEVLPEGKVDTVRQLQKQYGRLAFVGDGINDAPALAQADVGLAIGTGTDVAIEAADVVLMSGSLKGVPNAIALSKATMRNIRQNLFWAFVYNVALIPIAAGALYPAFGVLLSPMFAAGAMALSSVFVLGNALRLKRFHAPVH 3J09 Chain:A ((15-733)) ------------------------------------------------------------------------------------PMERTVRVTGMTCAMCVKSIETAVGSLEGVEEVRVNLATETAFIRFDEKRIDFETIKRVIEDLGYGV------VDEQAAVSAEVEHLSRMKRKLYVA---AFAGVLLLFLAHFISL----------PYEDFV-QLLIALPAIFYSGSSIFKAAFSALRRRTLNMDVMYSMGVGAAFLASVLST--AGVLPREYS--FYETSVLLLAFLLLGRTLEARAKSRTGEAIKKLVGLQAKTAVVIRDGKEIAVPVEEVAVGDIVIVRPGEKIPVDGVVVEGESYVDESMISGEPVPVLKSKGDEVFGATINNTGVLKIRATRVGGETLLAQIVKLVEDAMGSKPPIQRLADKVVAYFIPTVLLVAISAFIYWYFIAHAP-LLFAFTTLIAVLVVACPCAFGLATPTALTVGMGKGAELGILIKNADALEVAEKVTAVIFDKTGTLTKGKPEVTDLVPLNGDER-ELLRLAAIAERRSEHPIAEAIVKKALEHGIELGEPEKVEVIAGEGVVAD----GILVGNKRLMEDFGVAVSNEVELALEKLEREAKTAVIVARNGRVEGIIAVSDTLKESAKPAVQELKRMGIKVGMITGDNWRSAEAISRELNLDLVIAEVLPHQKSEEVKKLQAKE-VVAFVGDGINDAPALAQADLGIAVGSGSDVAVESGDIVLIRDDLRDVVAAIQLSRKTMSKIKQNIFWALIYNVILIPAAAGLLYPIFGVVFRPEFAGLAMAMSSVSVVANSLLLRNYVPPIR

General information: TITO was launched using: RESULT: Template: 3J09.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4067 -34779 -8.55 -48.78 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -8.55 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_3J09.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J09-query.scw PDB file : Tito_Scwrl_3J09.pdb: