Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSLKSLQCFVTLVKTKSFTRTAEELYLTQPTISKILQQLEEQLQVQLLVKPDHGRKRQIELTEIGERIYQHAGELLQAEQNIFLEIENYQQLKTGTLKLGVPPLGSQL--LTTALFDFHRQW-PDIELAFMEVGSRGIEQALLNNELDVGVLLQPFDEQTFNSIELCNYPLMVLLRRDA--TWATRKKINLEELQHQSFLMFPE----NFSLNSIILDACKQHGFYPTIACRTSQWHLLADMVLQRMGIALLPQYYTDMLDPTLFAAVPLEKPNIQWHLVMAWKKNLPVSPAVQAWLSIVRQHFQHIKP 3K1P Chain:A ((1-295)) -MELRHLRYFVAVVEEQSFTKAADKLCIAQPPLSRQIQNLEEELGIQLLERGS----RPVKTTPEGHFFYQYAIKLLSNVDQMVSMTKRIASVEK-TIRIGF--VGSLLFGLLPRIIHLYRQAHPNLRIELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKRTLLRNERLMVAVHASHPLNQMKDKGVHLNDLIDEKILLYPSSPKPNFSTH--VMNIFSDHGLEPTKINEVRKVQLALGLVAAGEGISLVPA-STQSIQLFNLSYVPLLDPDAITPIYIAV-RNMEESTYIYSLYETIRQIYAY---

General information: TITO was launched using: RESULT: Template: 3K1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1200 -70584 -58.82 -246.80 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -58.82 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_3K1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K1P-query.scw PDB file : Tito_Scwrl_3K1P.pdb: