Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIFSTNTCPVPNDLESVIHHKDEVPAELCGMSEQQVQKIWKSIESLYKTGNYPLITFCLRRQGKILLNRSIGYAQGNSPAGLQENALIATPDTPVCLFSASKMITAMLIHLLDEKGEINLLDPVSYYIPEY----------GVNG--------KRRATIFHLLSHRGGIPY---VDGDVTPELL--------FDKDEILRRLYAARPVSPAGNHLAYHAVTAGYILGEIIERVTGQDLRKFVHETIEKPMDMP----YFNYGLKPEYRAEVALNCATGLHPRLGTDHYLNHVLGGGLQLAVDVTNDSRFMDTICPAGN-IYTSAEQAGRFFEMLLSGGSYQGKQILSEKTIF--------RAT--------LPTTGVNIDRTLLIPMRYALGPMLGSNPVGLFGPMTGQAFGHLGFSNILCWADPERDISVSLLTTGKSVVGTHLPALANLLYQISIQCPRIPRDQRRSLFGSDSHETDLV 4IVK Chain:A ((37-421)) -----------------------------AGFTSEGLARIDAYLKNEIQAKTMPGAVMMIKRNGETAYFSSFGLRDPDTKEPM-------TAETIFRIYSMSKPITTVAAMMLVEEGKLQLDEPVSKYIPSFANVKVGVETKGENGMALETGPVKRAITIQDLMRHTSGITYGFVGDGLVKKAYIASNLFDGDFDNAEFAERIAKLPLVYQPGTTWDYGHSTD--ILGRVVEVVSGKSLYQFEKERLLDPLGMKDTGFYVTDPAKKSLVAEAMPN---------------DRKIGGSEMF------DPRVQKKWEPGGQGMVSTIGDYARFTQMVLNGGTLDGKRYLSPKTIAYMGSNHIPQASGIVPGAYYLPGPGVGFGLGFAVRTEAGVTPVEGSVGDLSWGGAGGTVF----------WIDPKENLTVVFMAPMVSPRARVWRTLRNIVY----------------------------

General information: TITO was launched using: RESULT: Template: 4IVK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1721 -24894 -14.46 -74.31 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -14.46 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_4IVK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IVK-query.scw PDB file : Tito_Scwrl_4IVK.pdb: