Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSYIAPVELEKAYRLLNHGPTVLVSAQHGDDRNVMAAAWACALEFKPAKVTVVLDKSTKTRQLVEQSGYFTLQVPCYAQLNMTKQLGIISKLDDPQKLEHCGVELFYQKDLTSPLVSGCIAWLVCKLIPEPHNQSAHDLFIGSVVGAWADSRVFRDGHWHFQDAPKELRSLHYIAGGTFYLIGEEVKADL 3ZOG Chain:A ((21-163)) ------------EGYRLLYPMRTYLIVSGHGEETNVMAADWVTVVSFDPFIVGVAVAPKRTTHKLIKKYGEFVISVPS---LDVLRDVWIAGTKKGPSKLKEMSVTLIPSKKVKVPSIEEALANIECRVI-DARSYGDHTFFVGEVVGYTYKDYAFEKG---------------------------------

General information: TITO was launched using: RESULT: Template: 3ZOG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 719 -99150 -137.90 -693.35 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -137.90 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_3ZOG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZOG-query.scw PDB file : Tito_Scwrl_3ZOG.pdb: