Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATMQCDVVSVKESLYSGAVTMLIAKGAGGELGILPGHAPLVTLLQLGPIRVLLENGTEEIVYVSGGVLEVQPHVVTVLADTAIRADNLDEAAILEARKNAEQLLANQKSDLDSAAALAALAETAAQLETIRKIKNRAQ 5T4P Chain:H ((4-137)) --TYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLTAIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKAEEHISSSHGDVDYAQASAELAKAIAQLRVIELTKK---

General information: TITO was launched using: RESULT: Template: 5T4P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 422 -60093 -142.40 -448.46 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain H : 0.86 3D Compatibility (PKB) : -142.40 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_5T4P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T4P-query.scw PDB file : Tito_Scwrl_5T4P.pdb: