Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQSVYHIPVKTISGETVDLDQYKGKVLLIVNTASKCGLTP-QYEGLEKLYQAKKDQGLEILGFPANNFKEQEPGSDEEIQQFCSLNYDVHFPLFSKISVAGEDKHPLYQALTTAQPERIGEGPFRERLEGLGIPTNPAPEVLWNFEKFLVNKNGEVIARFAPNLTADDEQIVKAVEAELAK 2OBI Chain:A ((11-155)) -ARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEF-AAGYNVKFDMFSKICVNGDDAHPLWKWMKI-QP------------KGKGILGNA---IKWNFTKFLIDKNGCVVKRYGP-------------------

General information: TITO was launched using: RESULT: Template: 2OBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 705 -11265 -15.98 -78.23 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -15.98 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_2OBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OBI-query.scw PDB file : Tito_Scwrl_2OBI.pdb: