Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATVDLFAIGNALIDQEFKVSDDFLSQQGLQKGTMQLSDGETQSALYEKLKQTQDYKGQASGGSAANTTVAFSALGGSAFYGCRVGHDELGGIYLQGLNDAGI--QTTPKSISEGVTGTCMVLISPDSERTMHTYLGITAELSQDQIDFEPLKTAKWLYIEGYLSTSETARKAVKQAREIAKAHGVKIALSLSDPAMVQYAREGLEELMDDG-VDLLFCNEQEALMYTNTTTVEDALTQLLFKNHTVVITQSAKGALVANPTHHFHVAGHHV-EAVDTNGAGDAFAGAFLYALNHHEDLTAAAQLAILISSEVVSQFGPRLAINDYAKLLENFQKECA 4JKU Chain:A ((27-337)) ----DVLTVGNAIVDIISRCNDQFLIDNQITKAAMNLIDAERAELLYSRMGPAL----EASGGSAGNTAAGVANLGGKAAYFGNVAADQLGDIFTHDIRAQGVHYQTKPKGAFP-PTARSMIFVTEDGERSMNTYLGACVELGPEDVEADVVADAKVTYFEGYLWDPPRAKEAILDCARIAHQHGREMSMTLSDSFCVDRYRGEFLDLMRSGKVDIVFANRQEALSLYQTDDFEEALNRIAADCKIAAVTMSENGAVILKGRERYYVNAIRIREVVDTTGAGDLFASGFLYGYTQGRSLEDCGKLGCLAAGIVIQQIGPR------------------

General information: TITO was launched using: RESULT: Template: 4JKU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1908 -32064 -16.80 -104.44 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -16.80 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4JKU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JKU-query.scw PDB file : Tito_Scwrl_4JKU.pdb: