Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPQYKAPLRDMQFVLHELLNAEEHYAKLPDFQGNVSRELVDQYLEAAANFCENELSPLNQVGDREGCTWNDGVVTTPTGFKEAYQKYIELGFPSLSAEEQYGGQGLPNSLGIAISEMVGSSNWAWGMYPGLSHGAVRT-LEHHGSEEQKNTYLPNLVSGVWTGTMCLTESHAGSDLGIIRTKAEPNADGSYAISGEKIFISAGEHDMAENIIHIVLARLPGAPKGTKGISLFIVPKFNVNADGTLGERNAVRCGSIEHKMGIHGNATCVINFDQA---KGYLIGPENRGLNCMFTFMNTARIGTAVQGLSASESSFQGALTYAKERLAMRSLSGPKAPEKEADPIIVHPAVRNMLLTQKAFAEGGRALVYLLAQYADIVEKGETEEERKFADNILSLLTPIAKAFLTETGSESAKHGVQVFGGHGFISEHGMEQIVRDTRIACLYEGTTEIQALDLLGRKVLQTQGAMLRDFTKIVHKFVEANKDNAAMKEFVEPLAALNKEWGDLTMQIGMRAMQNPDEVGAAAVDYLYFSGYVTLAYLWARMALVAQEKLAAGTTDVDFYNAKVTTARFYFKKILPRVRSHVDVIAGGLDPLMALDAEHFAF 4L1F Chain:A ((14-380)) --------------------------------------------DMAAEFAEKFLAPTVEERDK-AHIWDRKLI----------DKMGEAGFCGICFPEEYGGMGLDVLSYILAVEELSKVDDGTGITLSANVSLCATPIYMFGTEEQKQKYLAPIAEGTHVGAFGLTEPSAGTDASAQQTTAVLKGD-KYILNGSKIFITNGK----EADTYVVFA-MTDKSQGVHGISAFILEK------GMPG----FRFGKIEDKMGGHTSITAELIFEDCEVPKENLLGKEGEGFKIAMETLDGGRIGVAAQALGIAEGALAAAVKYSKER------------EQFGRSISKFQALQFMMADMATKIEAARYLVYHAAMLKN--------EGKPYSEA-----AAMAKCFASDVAMEVTTDAVQIFGGYGYTVDYPAERYMRNAKITQIYEGTNQVMR--------IVTSRALLRD-------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4L1F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2212 -46731 -21.13 -128.74 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -21.13 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_4L1F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4L1F-query.scw PDB file : Tito_Scwrl_4L1F.pdb: