Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTYAYVRIEPNLQFEFSRYISFFSEYGYKIQKNRLVVEEVSVDKSIVYRDKFLNLISYSLEEGDILVIKSMDCLGNSFEEILDIVNKIDQKKIRLICLDYS--KSEINGDLKIFFLHFLKLSAEFEKLFKSDKKNFKDHKP-------TRKVGRPEILNNEQKSRVIELFKKGYSVYSLAKEFSVTRTVIQRILNNASKTNIL 2GM4 Chain:A ((1-180)) MALFGYARVSTSQQ-SLDIQVRALKDAGVK--ANRIFTDKAS-------DRKGLDLLRMKVKEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDDSIDTSSAMGRMVVTILSAVA-QAERQRILQRTNEGRQEAMAKGVVFGRKRKIDRDAVLN---------MWQQGLGASHISKTMNIARSTVYKVIN--------

General information: TITO was launched using: RESULT: Template: 2GM4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 527 -68835 -130.62 -412.18 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -130.62 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_2GM4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM4-query.scw PDB file : Tito_Scwrl_2GM4.pdb: