Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASHYEMFPAVVTRVEQLTP---LIKRFTFKRQDGQNFPRFSGGSHIIVKMN-----EQLSNAYSLMSCTQDLSTYQVCVRKDVEGKGGSVFMHDQCNEGCEIQISEPKNLFPLAETGNKHI-LIAGGIGITPFLPQMDELAARG--AEYELHYAYRSPEHAALLDELTQ------KHAGHIF-----------SYVDSEGSMLNLDELISSQPKGT-HVYVCGPKPMIDAVIDCCNKHRYRDEYIHWEQFASTVPEDGEAFTVVLAKSNQEIEVQSNQTILQAIETLNIDVACLCREGVCGTCETAILEGEAEHFDQYLSDAEKASQKSMMICVSRAKGKKLVLDL 1GVH Chain:A ((156-391)) -------------RIVAKTPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSL-TRKPDGKGYRIAVKRE-EGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLAMLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAKGQFDSEG-LMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFG-----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 12566 11.70 60.71 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 11.70 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_1GVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GVH-query.scw PDB file : Tito_Scwrl_1GVH.pdb: